This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed.
URL of this page: How the Test is Performed Your health care provider will collect a sample of urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection.
The sample is then sent to a laboratory.
Some of the sample is placed on a glass slide, stained, and heated. The cells in the sample hold onto the dye. The slide is then washed with an acid solution and a different stain is applied. Bacteria that hold onto the first dye are considered "acid-fast" because they resist the acid wash.
These types of bacteria are associated with TB and other infections. How to Prepare for the Test Preparation depends on how the sample is collected. Your provider will tell you how to prepare.
How the Test will Feel The amount of discomfort depends on how the sample is collected. Your provider will discuss this with you. Why the Test is Performed The test can tell if you are likely infected with the bacteria that cause TB and related infections.
Normal Results A normal result means no acid-fast bacteria were found on the stained sample. Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples.
Talk to your doctor about the meaning of your specific test result. What Abnormal Results Mean Abnormal results may be due to: TB Nocardia infections also caused by a bacteria Risks Risks depend on how the sample is collected.
Ask your provider to explain the risks and benefits of the medical procedure. Acid-fast bacteria - culture and stain. Laboratory Tests and Diagnostic Procedures. The clinician and the microbiology laboratory.A Gram stain is a laboratory procedure used to detect the presence of bacteria and sometimes fungi in a sample taken from the site of a suspected infection.
It gives relatively quick results as to whether bacteria or fungi are present and, if so, the general type(s).
The Ziehl–Neelsen stain, also known as the acid-fast stain, was first described by two German doctors: the bacteriologist Franz Ziehl (–) and the pathologist Friedrich Neelsen (–).
It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. Buy Eosin-Wright's 2, Dip Stain, 16 oz (16 oz.): Chemicals & Water Testing Products - vetconnexx.com FREE DELIVERY possible on eligible purchases. Therefore, it is clinically important to be able to accurately identify members of these genera.
The acid-fast stain is a first step in identification of these types of bacteria.
Newcomer Supply AFB Stain, Kinyoun is used to demonstrate the presence of acid-fast mycobacteria in tissue sections. Phenol is employed to render the cell walls of bacteria permeable to the fuchsin stain. Acid-fastness is a physical property of certain bacterial and eukaryotic cells, The most common staining technique used to identify acid-fast bacteria is the Ziehl-Neelsen stain, in which the acid-fast species are stained bright red and stand out clearly against a blue background.